KRASNOYARSK, April 23 – RIA Novosti. Scientists of the Siberian Federal University (SFU) and the Institute of Biophysics SB RAS developed the concept of complex testing of agricultural land polluted with various chemicals due to the use of enzymes, it will more accurately analyze the soil, the press service of the SFU.
According to scientists, at present, to find the pollution used in agriculture soil and to take prompt remedial action, massively use biotests. For example, check the pollution of water or soil by using simple organisms – bacteria, algae or crustaceans-Daphnia. If their “mood” changes, it indicates the presence in the samples of hazardous substances. But the accuracy of such biotests low.
“Our research group has developed a comprehensive test system designed for environmental monitoring of soil. This enzyme biotests – more sensitive and accurate than the widely used standard biotests. They provide reliable results and are less dependent on random factors than, for example, tests with daphnids – by the way, no living thing in the result of our work has not suffered”, – quotes the press service of the Junior researcher of laboratory of bioluminescent biotechnology, SFU Elizabeth Kolosov.
it is Reported that for experiments, the scientists picked up chain enzymes that provide specific functions of a living organism – a single enzyme is responsible for respiration, the other involved in the process of digestion and so on. In the end, research shows that more than 10 such systems were selected for three enzyme system with maximum sensitivity to different classes of hazardous substances found in soil. It is important that when using all three systems in the complex is given is obtained as a complete list of harmful substances contained in the samples.
as model toxicants, researchers chose the main agricultural pollutants: phosphorous and organochlorine pesticides, nanoparticles of titanium dioxide (it is used, in particular, as a stimulator of plant growth) and copper chloride included in the composition of some pesticides. For experiments were selected five standard soil samples of sand, light loam, medium loam, heavy loam and black soil.
to exclude the influence of components of uncontaminated soil the results of testing, were used as a control aqueous extract from the soil without the addition of toxic substances.
